From GSA:

The GSA Image Analyser Batch Edition is a program for the scientific evaluation of images. The Program can analyse all images in batch mode and store the results in a CSV files who can later be imported into any spreadsheet applications like Excel or Open Office. The possibilities of the program are varied and can be divided in three main groups; Object surface calculation, Object length calculation and Object counting. Everything gets calculated automatically without any user input. The program offers the possibility to calculate the relations of the ascertained surfaces to each other and to the whole image or background. The GSA Image Analyser Batch Edition has also a set of functions which have been developed especially for the direct length calculation of very complex and very ramified objects (such objects are for example plant roots). There are also indirect calculation available with a grid intersection (developed by Tennant). The automatic count function determines the number of all recognised objects and, in addition, offers the possibility to count overlapping objects apart. This separation can made over the object form, the object surface and over the object intensity. The grid intersection count function serves for the indirect object number regulation. Such procedures are described, e.g., by Buerker, Fuchs Rosenthal, Thoma, shilling, Tuerk. The GSA Image Analyser Batch Edition can read almost all known picture formats (JEPEG, GIF, TIFF, BMP, PNG). This program is designed to solve many problems. It has been used for counting cells and calculating the size of plant roots (biometry).